HTLV-1 Tax Enhances NF-κB2 Expression and Binds to the Products p52 and p100, but Does Not Suppress the Inhibitory Function of p100
Identifieur interne : 004176 ( Main/Exploration ); précédent : 004175; suivant : 004177HTLV-1 Tax Enhances NF-κB2 Expression and Binds to the Products p52 and p100, but Does Not Suppress the Inhibitory Function of p100
Auteurs : Takashi Murakami [Japon] ; Hiroshi Hirai [Japon] ; Takeshi Suzuki [Japon] ; Jun-Ichi Fujisawa [Japon] ; Mitsuaki Yoshida [Japon]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: Tax protein of HTLV-1 triggers transcriptional activation through enhancers, NF-κB binding site, 21-bp enhancer, and serum response element. Previously, we demonstrated binding of Tax to transcription factors NF-κBt p105 and p50. Here, we report that Tax enhances expression of HF-κB2 at the mRNA level and proteins; the effect was more apparent on the p52 expression than on its precursor p100, suggesting post-translational regulation. Consistent with these observations, HTLV-1-infected T-cell lines expressed higher levels of p52. Tax binds to the protein products p52 and p100 which inhibits NF-κB proteins forming cytoplasmic complexes; the binding to p100 was preferential over NF-κB1 p105. However, Tax did not induce efficient dissociation of the cytoplasmic complexes p100/c-Rel or p100/p65, and thus did not induce nuclear translocation of c-Rel or p65. This was in sharp contrast to the previous observation that Tax dissociated the p105/c-Rel and IκB-γ/p65 complexes. These results indicate that HTLV-1 Tax interacts with NF-γB2 p100 and p52 and upregulate the NF-κB function, but their contribution to Tax-mediated transcriptional regulation differs from those of NF-κB1.
Url:
DOI: 10.1006/viro.1995.1029
Affiliations:
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from="1066">1066</biblScope><biblScope unit="page" to="1074">1074</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Tax protein of HTLV-1 triggers transcriptional activation through enhancers, NF-κB binding site, 21-bp enhancer, and serum response element. Previously, we demonstrated binding of Tax to transcription factors NF-κBt p105 and p50. Here, we report that Tax enhances expression of HF-κB2 at the mRNA level and proteins; the effect was more apparent on the p52 expression than on its precursor p100, suggesting post-translational regulation. Consistent with these observations, HTLV-1-infected T-cell lines expressed higher levels of p52. Tax binds to the protein products p52 and p100 which inhibits NF-κB proteins forming cytoplasmic complexes; the binding to p100 was preferential over NF-κB1 p105. However, Tax did not induce efficient dissociation of the cytoplasmic complexes p100/c-Rel or p100/p65, and thus did not induce nuclear translocation of c-Rel or p65. This was in sharp contrast to the previous observation that Tax dissociated the p105/c-Rel and IκB-γ/p65 complexes. These results indicate that HTLV-1 Tax interacts with NF-γB2 p100 and p52 and upregulate the NF-κB function, but their contribution to Tax-mediated transcriptional regulation differs from those of NF-κB1.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Murakami, Takashi" sort="Murakami, Takashi" uniqKey="Murakami T" first="Takashi" last="Murakami">Takashi Murakami</name></region><name sortKey="Fujisawa, Jun Ichi" sort="Fujisawa, Jun Ichi" uniqKey="Fujisawa J" first="Jun-Ichi" last="Fujisawa">Jun-Ichi Fujisawa</name><name sortKey="Hirai, Hiroshi" sort="Hirai, Hiroshi" uniqKey="Hirai H" first="Hiroshi" last="Hirai">Hiroshi Hirai</name><name sortKey="Suzuki, Takeshi" sort="Suzuki, Takeshi" uniqKey="Suzuki T" first="Takeshi" last="Suzuki">Takeshi Suzuki</name><name sortKey="Yoshida, Mitsuaki" sort="Yoshida, Mitsuaki" uniqKey="Yoshida M" first="Mitsuaki" last="Yoshida">Mitsuaki Yoshida</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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